The Beckman Center for Cryo-EM at Johns Hopkins was established to provide cutting edge cryo-EM to the Johns Hopkins and regional communities. The heart of the center is the G3 Titan Krios® equipped with a phase plate and K3® Gatan direct detector. The center also maintains various support equipment such as a FEI Vitrobot® Mark IV and Pelco easiGlow®. Modern facilities, such as this one when fully completed, are capable of producing thousands of high resolution cryo-EM images or scores of tomogram tilt series per day. These images allow the determination of high resolution cellular component structures, up to ~2Å.
Technical assistance is provided upon request. Training is available for all levels of user experience on an as needed basis.
The Beckman Center was made possible through generous grants from the Arnold and Mabel Beckman Foundation, the Johns Hopkins School of Medicine, and private donors. Operations support provided through user fees and Johns Hopkins.
In addition to the physical instrumentation, the Beckman Center funding plan includes support for a technical operations director and a cryo-EM faculty member.