November 6, 2025 kcai11

September 2025 Microscope Status Update

September’s Krios ApoF structure @ 1.9 Å resolution

Pixel size 0.93 Å (130kx)
1587/1729 exposures used (CTF fit 0-5 Å)
544,898/570,622 particles used
NCC score of 0.54

September’s Glacios ApoF structure @ 2.73 Å resolution

Pixel size 1.1896 Å
1466/1564 exposures used (CTF fit 0-5 Å)
712,379/796,924 particles used
NCC score of 0.497

The result on Glacios is 0.4 Å off from what it should be. We noticed that the roof chiller issue, which occurred several days before benchmarking, may have affected the alignment. We are working on it to restore the Glacios to optimal.