SCHEDULING EQUIPMENT CryoEM
  @ Hopkins
Titan Krios
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Beckman Center for CryoEM at John Hopkins

The Beckman Center for Cryo-EM at Johns Hopkins provides cutting edge cryo-EM to the Johns Hopkins and regional communities. Located in the Rangos building on the Johns Hopkins University School of Medicine campus, the center houses a 300 kV G3 Titan Krios TEM, along with a 200 kV Glacios TEM and an Aquilos II for FIB-SEM and cryo-CLEM. The Beckman Center is equipped for all aspects of high-resolution single-particle cryo-EM and tomography data collection, as well as preparation of samples for both single-particle cryo-EM and tomography.

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Recent News

April 2025 Microscope Status Update

April’s Krios ApoF structure @ 1.90 Å resolution

Pixel size 0.93 Å
678/732 exposures used (CTF fit 0-5 Å)
401,334/562,280particles used
NCC score of 0.300

April’s Glacios ApoF structure @ 2.43 Å resolution

Pixel size 1.19 Å
852/1606 exposures used (CTF fit 0-5 Å)
520,499/593,384 particles used
NCC score of 0.414

Uncategorized

March 2025 Microscope Status Update

March’s Krios ApoF structure @ 1.90 Å resolution

Pixel size 0.93 Å
1008/1032 exposures used (CTF fit 0-5 Å)
571,525/591,419 particles used
NCC score of 0.432

March’s Glacios ApoF structure @ 2.43 Å resolution

Pixel size 1.19 Å
529/1016 exposures used (CTF fit 0-5 Å)
371,352/486,892 particles used
NCC score of 0.42

Uncategorized

February 2025 Microscope Status Update

February’s Krios ApoF structure @ 1.90 Å resolution

Pixel size 0.93 Å
1074/1128 exposures used (CTF fit 0-5 Å)
635,484/675,928 particles used
NCC score of 0.39

February’s Glacios ApoF structure @ 2.43 Å resolution

Pixel size 1.19 Å
624/1153 exposures used (CTF fit 0-5 Å)
488,455/599,011 particles used
NCC score of 0.44

Uncategorized

Status

Titan Krios: Up

Glacios: Up

Aquilos: Up

Vitrobot 1 (PCTB): Up

Vitrobot 2 (Rangos): Up

<size=32>CryoEM at Johns Hopkins Medicine</size>
<size=32>CryoEM at Johns Hopkins Medicine</size>

Our Equipment

Aquilos II

Aquilos II

Instruments
EM ACE 600

EM ACE 600

Support Equipment
Glacios

Glacios

Instruments
Pelco easiGlow

Pelco easiGlow

Support Equipment
Titan Krios

Titan Krios

Instruments
Vitrobot Mark IV

Vitrobot Mark IV

Support Equipment