SCHEDULING EQUIPMENT CryoEM
  @ Hopkins
Titan Krios
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Beckman Center for CryoEM at John Hopkins

The Beckman Center for Cryo-EM at Johns Hopkins provides cutting edge cryo-EM to the Johns Hopkins and regional communities. Located in the Rangos building on the Johns Hopkins University School of Medicine campus, the center houses a 300 kV G3 Titan Krios TEM, along with a 200 kV Glacios TEM and an Aquilos II for FIB-SEM and cryo-CLEM (to be installed late 2023). The Beckman Center is equipped for all aspects of high-resolution single-particle cryo-EM and tomography data collection, as well as preparation of samples for both single-particle cryo-EM and tomography.

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Recent News

January 2025 Microscope Status Update

January’s Krios ApoF structure @ 1.90 Å resolution

Pixel size 0.93 Å
701/864 exposures used (CTF fit 0-5 Å )
249,958/339,960 particles used
NCC score of 0.45

December’s Glacios ApoF structure @ 2.43 Å resolution

Pixel size 1.19 Å
582/921 exposures used (CTF fit 0-5 Å )
412,001/501,206 particles used
NCC score of 0.4

Uncategorized

December 2024 Microscope Status Update

December’s Krios ApoF structure @ 1.90 Å resolution

Pixel size 0.93 Å
752/840 exposures used (CTF fit 0-5 Å )
363,991/381,781 particles used
NCC score of 0.485

December’s Glacios ApoF structure @ 2.43 Å resolution

Pixel size 1.19 Å
647/814 exposures used (CTF fit 0-5 Å )
525,444/584,015 particles used
NCC score of 0.452

Uncategorized

November 2024 Microscope Status Update

November’s Krios ApoF structure @ 1.92 Å resolution

Pixel size 0.93 Å
772/819 exposures used (CTF fit 0-5 Å )
421,654/439,133 particles used
NCC score of 0.46

November’s Glacios ApoF structure @ 2.43 Å resolution

Pixel size 1.19 Å
674/807 exposures used (CTF fit 0-5 Å )
577,594/844,920 particles used
NCC score of 0.3

Uncategorized

Status

Titan Krios: Up

Glacios: Up

Aquilos: Installation completed

Vitrobot 1 (PCTB): Up

Vitrobot 2 (Rangos): Up

<size=32>CryoEM at Johns Hopkins Medicine</size>
<size=32>CryoEM at Johns Hopkins Medicine</size>

Our Equipment

Aquilos II

Aquilos II

Instruments
EM ACE 600

EM ACE 600

Support Equipment
Glacios

Glacios

Instruments
Pelco easiGlow

Pelco easiGlow

Support Equipment
Titan Krios

Titan Krios

Instruments
Vitrobot Mark IV

Vitrobot Mark IV

Support Equipment