Equipment

February 2024 Microscope Status Update

Titan is back online with a repaired energy filter. Resolution at 130kx is 1.89A with 200k particles. 215kx is 1.78A with 140k particles. Aquilos installation is completed, and workflow validation is scheduled for the week of Feb 26, followed by staff training.

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January 2024 Microscope Status Update

We performed a benchmarking on January 10 for the Titan Krios. At magnification 130kx, we collected 954 exposures and used 690 with a 7A CTF filter. We used 288140/525273 particles and obtained a resolution of 1.89A. The pixel size is around 0.93A. The Glacios benchmarking was done on January 22. At magnification 120kx, we collected 753 exposures and used 520…

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December 2023 Microscope Status Update

We performed a benchmarking on November 19 for the Titan Krios. At magnification 130kx, we collected 852 exposures and used 409 with a 4A CTF filter. We used 108190/200249 particles and obtained a resolution of 1.90A. The pixel size is around 0.93A. The Glacios benchmarking was done on November 22. At magnification 120kx, we collected 596 exposures and used 446…

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October 2023 Microscope Status Update

We performed a benchmarking on October 17 for the Titan Krios. At magnification 130kx, we collected 1493 exposures and used 965 with a 4A CTF filter. We used 444668/533716 particles and obtained a resolution of 1.90A. The pixel size is around 0.93A. The Glacios benchmarking was done on October 31. At magnification 120kx, we collected 599 exposures and used 227…

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September 2023 microscope status update

We performed a benchmarking on September 14 for the Titan Krios. At magnification 165kx, we collected 1133 exposures and used 856 with a 5A CTF filter. We used 209094/348665 particles and obtained a resolution of 1.76A. The pixel size is around 0.74A. The Glacios benchmarking was done on September 28. At magnification 120kx, we collected 983 exposures and used 296…

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August 2023 microscope status update

We performed another benchmarking on August 17 for the Titan Krios. At magnification 130kx, we collected 717 exposures and extracted 209021 particles. Cryosparc gives a resolution of 1.93A. The pixel size is around 0.93A. We believe the Nyquist is reached. At magnification 165kx, we collected 992 exposures and extracted 108962 particles. Cryosparc gives a resolution of 2.01A. The pixel size…

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