Titan is back online with a repaired energy filter. Resolution at 130kx is 1.89A with 200k particles. 215kx is 1.64A with 120k particles. Glacios is working well. Resolution at 120kx is 2.43A with 202k particles. 240kx is 2.23A with 99k particles. Aquilos installation is completed, and workflow validation is scheduled for the week of Feb 26, followed by staff training.
We performed a benchmarking on January 10 for the Titan Krios. At magnification 130kx, we collected 954 exposures and used 690 with a 7A CTF filter. We used 288140/525273 particles and obtained a resolution of 1.89A. The pixel size is around 0.93A. The Glacios benchmarking was done on January 22. At magnification 120kx, we collected 753 exposures and used 520…
We performed a benchmarking on November 19 for the Titan Krios. At magnification 130kx, we collected 852 exposures and used 409 with a 4A CTF filter. We used 108190/200249 particles and obtained a resolution of 1.90A. The pixel size is around 0.93A. The Glacios benchmarking was done on November 22. At magnification 120kx, we collected 596 exposures and used 446…
We performed a benchmarking on October 17 for the Titan Krios. At magnification 130kx, we collected 1493 exposures and used 965 with a 4A CTF filter. We used 444668/533716 particles and obtained a resolution of 1.90A. The pixel size is around 0.93A. The Glacios benchmarking was done on October 31. At magnification 120kx, we collected 599 exposures and used 227…
We are excited to announce the appointment of Dr. Kai Cai as the Cryo-EM Specialist at the Beckman Center in October 2023. Kai worked with SPA and tomography at the University of Wisconsin-Madison in the past few years. She will operate the facility with her experience and knowledge in electron microscopy. The facility committees and users welcome Kai’s arrival and…
We performed a benchmarking on September 14 for the Titan Krios. At magnification 165kx, we collected 1133 exposures and used 856 with a 5A CTF filter. We used 209094/348665 particles and obtained a resolution of 1.76A. The pixel size is around 0.74A. The Glacios benchmarking was done on September 28. At magnification 120kx, we collected 983 exposures and used 296…